Longevity Questions

20 level 8 questions for the first question of the seventh level ("What is the complete three-dimensional atomic structure of NADPH oxidase and its catalytic centers?")
1. What methods are the most accurate for obtaining the atomic structure of NADPH oxidase?
2. What are the exact coordinates of the atoms in the active center of NADPH oxidase?
3. What amino acid residues form the active site of NADPH oxidase?
4. What is the structure of the NADPH oxidase domains responsible for binding NADPH?
5. How is the electron transfer channel structurally organized within NADPH oxidase?
6. What protein interactions stabilize the three-dimensional structure of NADPH oxidase?
7. How does NADPH oxidase structurally change upon binding of the substrate (NADPH)?
8. What is the exact structure of the membrane domain of NADPH oxidase?
9. What structural elements of NADPH oxidase are responsible for the specificity of oxygen binding?
10. What atomic structures provide the interaction of NADPH oxidase with regulatory proteins?
11. How does the lipid environment affect the three-dimensional structure of NADPH oxidase?
12. How do structural defects affect the activity of the catalytic centers of NADPH oxidase?
13. What structural changes in NADPH oxidase are necessary for its activation?
14. What is the structure of the NADPH oxidase complex in the inactive state?
15. What atomic interactions stabilize the intermediate states of NADPH oxidase?
16. How are metal-containing centers (e.g., hemes) structurally organized in NADPH oxidase?
17. How does the structure of NADPH oxidase change during redox reactions?
18. How does the atomic structure of NADPH oxidase interact with inhibitors?
19. What are the structural elements responsible for the assembly of NADPH oxidase into a functional complex?
20. How accurate are current methods for modeling the atomic structure of NADPH oxidase?
20 Level 9 questions for the first question of Level 8 (“Which methods are most accurate for obtaining the atomic structure of NADPH oxidase?”):
1. What are the advantages and limitations of X-ray crystallography for obtaining the atomic structure of NADPH oxidase?
2. Can cryo-electron microscopy (cryo-EM) provide atomic resolution of NADPH oxidase, and what is the optimal procedure?
3. Which method provides the best detail of the active site structure of NADPH oxidase?
4. What are the challenges in crystallizing membrane proteins such as NADPH oxidase?
5. What ancillary methods (e.g. NMR spectroscopy) can be used to clarify the structure of NADPH oxidase?
6. What are the requirements for NADPH oxidase samples to obtain an atomic-resolution structure?
7. Which approach is more effective for studying conformational changes in NADPH oxidase: cryo-EM or X-ray crystallography?
8. Which computer modeling methods most accurately predict the atomic structure of NADPH oxidase?
9. How does the quality and purity of the NADPH oxidase preparation affect the accuracy of its atomic structure?
10. What specialized sample preparation protocols are needed to obtain high-quality NADPH oxidase structures using cryo-EM?
11. Which data reconstruction and processing algorithms are most effective in determining the atomic structure of NADPH oxidase?
12. What additional experimental techniques help confirm the accuracy of the obtained NADPH oxidase structure?
13. How can the accuracy of the obtained atomic structure of NADPH oxidase be verified experimentally?
14. What conditions (temperature, pH, ionic strength) are optimal for stabilizing the NADPH oxidase structure during experiments?
15. How can the resolution of NADPH oxidase structures obtained using cryo-EM be improved?
16. How does the choice of detector and equipment affect the final accuracy of the NADPH oxidase structure?
17. What methods of protein labeling or stabilization can help to improve the accuracy of NADPH oxidase structures?
18. What approaches to data processing and filtering are needed to ensure the accuracy of the obtained atomic models of NADPH oxidase?
19. What modern methods allow one to simultaneously study the structure and dynamics of NADPH oxidase?
20. What are the prospects for using neural network methods (e.g., AlphaFold) for accurate modeling of the atomic structure of NADPH oxidase?