Longevity Questions

Perpetual Prompt ♾️, [08.05.2025 14:46]
401. Is it possible to create a self-regulating community of intestinal bacteria using synthetic ecology, constantly maintaining an anti-inflammatory metabolite profile without external interventions?

402. At what critical telomere length (in kilobases) do CD8⁺‑T cells lose the ability to clonal expansion after vaccination?

403. Which miRNA cocktail (miR‑155, miR‑17‑92, miR‑181a) is able to restore the proliferation of short-telomeric CD8⁺‑T cells without oncogenic transformation?

404.What protocol combining transient hTERT mRNA and PD-1/LAG-3 checkpoint modulation minimizes tumor risk during T cell rejuvenation?

405.What might a service model of periodic TCR repertoire “reset” using rejuvenated progenitors look like to support human immunity for two centuries?

406.At what age does FOXN1 expression in thymic epithelial cells fall below the threshold for naïve T cell production?

407.Does single AAV delivery of FOXN1 and IL-7 restore functional naïve T cell output in 60-year-old patients to levels typical of 30-year-olds?

408.What is the risk of autoimmune and allergic reactions after thymic rejuvenation based on co-expression of FOXN1 and IL-7 in elderly people?

409.Is it possible to ensure stable functioning of the thymus until the age of 150 without repeated interventions using CRISPR activation of endogenous FOXN1 enhancers?

410.In which human tissues (skin epithelium, hematopoietic system, hypothalamus) is the earliest and fastest acceleration of the DNA methylome clock observed after puberty?

411.What dynamic changes in growth hormones, IGF-1 and sex steroids directly trigger the indicated acceleration of the epigenetic clock in the selected tissue?

412. Can local CRISPR interference of TET dioxidases in skin epithelium stop the progression of biological age of this epithelium without systemic side effects?

413. How safe are multiple cycles of partial reprogramming (OSK factors) for global rejuvenation of the human epigenome without the risk of tumor formation and loss of cellular memory?

414. By what percentage does the secretion of Wnt7a and GDF11 by bone marrow mesenchymal stem cells decrease between the ages of 40 and 70?

415. Does forced expression of Wnt7a and GDF11 in mesenchymal stem cells restore muscle and bone tissue regeneration in old mice to the level of young mice?

416.What is the incidence of fibrosis and sarcomas in animals receiving quarterly infusions of genetically modified mesenchymal stem cells for ten years?

417.Is it possible to create an implantable “smart” bioreactor from mesenchymal stem cells that releases regenerative factors only in response to local pro-inflammatory signals?

418.What combination of aging biomarkers and economic indicators would allow biological aging to be classified as a disease in the WHO International Classification of Diseases (ICD)?

419.What is the expected gain in QALYs and reduction in medical costs in the USA, Japan, Germany, Brazil and India if aging were officially recognized as a disease?

420.What insurance reimbursement models (pay-for-results, subscription, bundledcare) would provide coverage for proven anti-aging therapies after aging is included in the ICD?

421. How will the distribution of investment in pharmaceutical R&D change over the 20 years following the official recognition of aging as a disease?

422. What proportion of the population in the United States, China, India, Germany, and Nigeria considers radical life extension to be ethically acceptable, according to recent representative surveys?

423. What are the main religious and philosophical narratives that shape resistance to the idea that human death is a surmountable technical problem?

424. What communication strategies (the “healthy longevity” frame, positive examples, storytelling) are most effective in reducing cultural resistance to radical life extension?