Longevity Questions

Perpetual Prompt ♾️, [08.05.2025 22:04]
781. How much does the rate of accumulation of plasma AGE modifications (glycated end products) decrease with a daily intake of 250 mg of gamma-aminoguanidine in healthy people aged 40–50 years — as a percentage of the control after 12 months?
782. How much does DNA methylation epigenetic age (according to Clock Grim Age) decrease after a nine-day course of periodic cis-plasma autoplasma exchange (1.5 plasma volumes per session) in people aged 60–65 years?
783. Does the effect from question 782 persist after 18 months without repeated procedures (yes/no)?
784. By what percentage does the rate of telomeric shortening in T-hCD4 lymphocytes decrease with 400 mg of cyclic GDF11 mimetic once a week for a year?
785. Does twice-annual stimulation of the thymus with rhGH (0.015 mg/kg/day) reduce the age-adjusted risk of pneumonia death by ≥20% (yes/no)?
786. Does permanent CRISPR suppression of the PCSK9 gene reduce apoB lipoprotein levels below 30 mg/dL in >95% of patients after 24 months?
787. Does transient integration of P16-INK4a-shRNA in ubiquitous keratinocytes (cutaneous injection) cause a 50% increase in local SC proliferation (EPC–Ki67+) in 14 days?
788. What micromolar threshold is required for plasma phenolspermidine levels to exceed 2.5 years in 40 days in the in vitro FibroAge assay?
789. Does inhibition of LINE-1 expression (anti-L1-Gapmer) in HSC culture suppress >90% STING signal activation in 48 hours (yes/no)?
790. Does weekly infusion of NAD+ (750 mg) prolong the length of the slow-wave-sleep phase space by ≥15% in individuals aged 50+ after one month?
791. What percentage of β-oxidatively lost mitochondria returns to normal membrane potential after a 14-day course of selective Urolithin-A 500 mg/day?
792. Does intracranial deep stimulation of the thalamic-mediodorsal nucleus (130 Hz, 60 µs) reduce the incidence of delirium after surgery in patients aged 70+ by ≥40%?
793. Does daily intake of 10 mg of the ACT inhibitor everolimus reduce the proportion of age-related clones (>0.02 VAF) in peripheral blood for 12 months (yes/no)?
794. Does a daily intake of 100 billion CFU of Akkermansia mucinifila reduce the plasma level of the indica to below 2 µmol/L in 6 weeks?
795. How many hours of high-intensity-interval-training (HIIT, VO₂max>85%) are required per week to reduce cardiorespiratory fitness age by ≥5 years in six months?
796. Combination of quercetin + dastinib: does it reduce the number of SA-β-Gal+ cells in subcutaneous adipose tissue in people aged 55-70 years by ≥25% after 1 month?
797. Is the effect of question 796 maintained after 12 months without repeat therapy (yes/no)?
798. What is the minimum level of β-hydroxybutyrate (mmol/L) required for a 15% reduction in marker expression of NLRP3 in monocytes in vivo?
799. At what threshold reduction in blood serine glycoperoxidase levels (<X U/L) does the rate of formation of covalent collagen cross-links in tendons decrease by ≥30%?
800. Does monthly subcutaneous implantation of IL-2 mimetic microarray pump (0.33 ml/day) reduce the proportion of CD28 negative CD8+ T cells to 10% of the T cell pool?
801. How many weeks of continuous Y-amanaka reprogramming therapy (OSK mRNA, pulse 1×4 days) are needed for the epigenetic age of the human liver to decrease by >10 years Ex-vivo?
802. What percentage does the plasma lipofuscin level decrease after a two-month course of alpha-cucurbitin 600 mg/day in people 60+?
803. Does MAPK-p38 activity in dermal fibroblasts decrease by >35% 24 hours after SINGLE topical application of astaxanthin-nanoliposomes (yes/no)?
804. Does optimization of the depth of meditation practice (60 min audio-encephalo-feedback, gamma 40 Hz) cause a decrease in the rate of cortical thinning of the prefrontal cortex by ≥0.02 mm/year?
805. What percentage of AGS mice will survive up to 400 days if they are suppressed by TGF-β antibodies with 1 mg/kg once a week from the age of two months?
806. How many years should the Klotho-modified AAV9-hTTR (liver) vector be expressed before overall mortality is reduced by ≥20% in a cohort model?

Perpetual Prompt ♾️, [08.05.2025 22:04]
807. Does monthly dental photodisinfection (405nm) suppress the growth of Porphyromonas gingivalis to <10³ CFU/mL after 6 months?
808. How much peak irradiance (630nm vs 810nm) of LLT is needed to increase Neprilysin activity by 50% in cultured glial cells?
809. Will selective inhibition of PDE11A4 (PF-04447943 inhibitor, 5 mg/day) increase the sensitivity of circadian period to melatonin by ≥25% (assessed through DLMO shift)?
810. Does a single intravenous administration of 2×10⁸CD47-negative mesenchymal SC reduce serum TNF-α and IL-6 by >50% after 48 hours?