Longevity Questions

Perpetual Prompt ♾️, [08.05.2025 22:27]
871. What specific genes in hibernating mammals are responsible for cellular resistance to prolonged hypothermia and can they be expressed in human tissues?

872. Is there a temperature threshold at which human metabolism can be stably suppressed without irreversible damage to neurons?

873. What substances are currently the most effective cryoprotectants for preventing intracellular crystallization in primates?

874. What level of oxygen is required to maintain the viability of neurons under hypothermia at 25°C for more than 12 hours?

875. Is it possible to achieve metabolic “stop mode” in human cells without cooling, only through biochemical regulation?

876. Which cryoprotective proteins found in insects and fish have been successfully synthesized in the laboratory and tested on human tissues?

877. Is it possible to induce positive systemic restructuring of the immune response in humans using short-term hypothermia (less than 6 hours)?

878. What minimum set of enzymes is needed to maintain DNA repair in cells when cooled below 10°C?

879. Which types of neurons in the cerebral cortex are most resistant to hypoxia during hypothermic slowing of blood circulation?

880. What is the maximum shelf life of human cells at -130°C without losing their ability to replicate?

881. Is it possible to modify human mitochondrial metabolism so that it goes into anabiosis mode, similar to tardigrades?

882. What is the minimum amount of energy required to maintain reversible anabiosis in humans for 30 days?

883. Which neuroprotectors have proven effective in preserving the synaptic structure of the brain during hypothermia?

884. What cooling rate is safe for the human brain to 5°C to avoid the formation of ice microcrystals?

885. Is it possible to replace cryoprotectors with polymer gels with antifreeze properties that are biocompatible with human tissue?

886. What are the biomarkers of reversibility of the hypothermic state in real time in humans?

887. What changes in the epigenome occur during prolonged cooling of tissues, and can they be used to stabilize cellular youth?

888. Which microcirculation models provide the best tissue recovery after hypothermic anabiosis?

889. Are there differences in resistance to cryodamage between human embryonic stem cell lines?

890. What are the minimum environmental conditions required for brain survival at -10°C for 24 hours?

891. What is the critical Na⁺/K⁺ ratio in neurons to trigger irreversible processes during hypothermia?

892. Is it possible to induce reversible hypothalamic depression in humans to simulate hibernation?

893. What is the metabolite profile observed during recovery from hibernation in mice and can it be verified in primates?

894. Which human tissues are most vulnerable to degradation after thawing with current cryopreservation methods?

895. Can CRISPR be used to turn on hibernation genes in humans without triggering an autoimmune response?

896. What signaling pathways are activated in the tardigrade brain when metabolism slows down, and is it possible to artificially activate them in mammals?

897. What pH gradients are safest for preserving neuroplasticity under conditions of artificial anabiosis?

898. Is it possible to preserve the integrity of the brain's glial network at liquid nitrogen temperatures if apoptosis inhibitors are used beforehand?

899. What proteins are key to synapse restoration after prolonged hypothermia, and how can their expression be enhanced?

900. What combinations of temperatures, cryoprotectants, and restorative procedures ensure maximum preservation of cognitive function after cryosleep?