Longevity Questions

Perpetual Prompt ♾️, [08.05.2025 23:59]
951. Does weekly feeding of donor rats with a diet rich in α-ketoglutarate reduce the level of accumulated DSBs in their HSCs to the youth background?
952. What is the maximum level of a clone with the JAK2V617F mutation (in %) that is acceptable in the pool of autologous HSCs so that the risk of post-transplant myeloproliferation remains <1%?
953. Is it possible to erase the age-associated H3K4me3 mark on the promoters of myelopoiesis genes with an HDAC inhibitor without suppressing lymphopoiesis (yes/no)?
954. Is NRF2 signaling in donor HSCs sufficient to restore telomere length by >1 kb in 4 weeks in vivo?
955. How long (hours) does ^89^Zr-oxymedronyl-PET take to non-invasively track homing of ≥90% of infused HSCs into the femoral head?
956. Does daily metformin (1 g/day) after HSC transplantation reduce the risk of GC-induced insulin resistance to that of young controls?
957. Do rejuvenated HSCs in aged mice improve maximal rotarod retention time by >25% compared to controls?
958. Does the combined use of young HSCs and recombinant IL-7 restore the CD4:CD8 ratio in aged primates to ≥1.5 within 6 months?
959. What is the minimum number of targeted deep sequencing points required to detect ASXL1 mutations in the donor HSC pool with a threshold of 0.01%?
960. Can a one-time local delivery of a biosoluble scEM hydrogel loaded with young HSCs increase trabecular bone mineral density in aged mice by ≥15% within 8 weeks?

Perpetual Prompt ♾️, [09.05.2025 00:01]
961. Will a one-time systemic delivery of CRISPR-Cas13d, which suppresses p16^INK4a expression, reduce the proportion of SA-β-galacto-positive cells in the skin of 35-year-old volunteers by ≥70% after 6 months?

962. Will daily administration of 10 mg of astraposin for 24 months increase the average leukocyte telomere length in 50-60-year-old humans by at least 1 kb compared to placebo?

963. Will infusion of 10^9 universal iPSC-derived thymic epithelial cells restore thymus volume in 60-year-old men to ≥20 cm³ after 12 months?

964. Will monthly dasatinib + quercetin senotherapy for 18 months reduce the epigenetic age (Horvath Clock) in 55-year-old women by ≥5 years?

965. Will AAV-Myc/TERT delivery to the liver of 40-year-old macaques accelerate the increase in maximum metabolic rate by ≥15% without inducing tumors over 3 years of follow-up?

966. Will a single transplant of young microbiome from 20-25-year-old donors reduce TMAO levels in 65-year-old recipients by ≥40% over 6 months?

967. Will weekly infusion of plasma from ≤25-year-old donors for 1 year increase the lifespan of NIA UM-HET3 mice by at least 15%?

968. Will 2-week dCas9-SAM expression of OSK in the retina of 45-year-old patients suppress ≤0.1 units of visual acuity loss over 5 years?

969. Will a 1-month course of a LINE-1 RT inhibitor reduce cytosolic cGAS activation in muscle biopsies in 70-year-olds by ≥50%?

970. Will 12-week combination therapy with metformin + intermittent fasting reduce plasma GDF15 levels in prediabetics 45–55 years by ≥30%?

971. Will mandatory insurance coverage of geroprotective procedures in country X increase the willingness of the 35–50 year-old population to undergo prophylactic courses by ≥25 p.p.?

972. Will a ban on smoking in public places reduce the 8-OxoG level in the blood of city residents aged 40–60 by ≥20% in 2 years?

973. Will annual screening of epigenetic age among enterprise employees increase participation in healthy lifestyle programs by ≥40%?

974. Will the introduction of a 4-day workweek reduce the chronic stress indicator (cortisol in saliva) in workers aged 30–45 by ≥25% in six months?

975. Will mandatory iodine and selenium fortification of table salt reduce the incidence of subclinical hypothyroidism in women aged 50+ to <5%?

976. Will a government tax deduction for sports activities increase the average weekly physical activity of the population aged 18–65 to ≥150 minutes?

977. Will the demonstration of personalized “organ age” (MRI biomarkers) convince ≥60% of patients aged 40–55 to switch to a Mediterranean diet?

978. Will the introduction of a digital sleep tracker in corporate insurance reduce the number of nights with <6 hours of sleep in IT employees by ≥30% over 1 year?

979. Will mandatory stress management training in schools increase the proportion of graduates without clinical anxiety to ≥85%?

980. Will free distribution of fitness trackers in city Y reduce the average BMI of residents aged 30–50 by ≥1 kg/m² in 24 months?

981. Will using a GPT assistant for planning meals reduce daily added sugar intake in users aged 25–45 by ≥20% in 3 months?

982. Will annual CMV booster vaccination reduce the number of CD28-null T cells in individuals aged 60+ to levels of <10%?

983. Will regular 30-minute aero-cryotherapy accelerate the recovery of mitochondrial potential in the muscles of veteran runners by ≥15%?

984. Will constantly wearing a smart posture correcting bracelet reduce the incidence of low back pain episodes in office workers by ≥50% per year?

985. Will a two-year program to replace carbonated beverages with water reduce the risk of insulin resistance in adolescents aged 12–16 years by ≥30%?

986. Will a decentralized blockchain registry system for biomedical data reduce the clinical trial time of geroprotective drugs from 7 to ≤4 years?

987. Will a mass transition to HEPA filtration in schools reduce PM2.5 concentrations in classrooms to <5 μg/m³, reducing the incidence of asthma attacks in children by ≥40%?

Perpetual Prompt ♾️, [09.05.2025 00:01]
988. Will monthly sclerostin monomer therapy increase trabecular bone density in postmenopausal women by ≥5% over 18 months?

989. Will implantable glucose microsensors with automated microdosing of GLP-1 reduce glucose variability (CV) in patients with prediabetes to <15%?

990. Will the combination of 20 minutes of daylight (10,000 lux) and 0.3 mg melatonin before bed reduce sleep onset latency in people aged 60+ to <15 minutes?