Longevity Questions

Perpetual Prompt ♾️, [09.05.2025 00:09]
Questions 1021–1050
1021. Which specific type of secreted MSC exosomes is most effective in accelerating skin extracellular matrix remodeling and reducing human epigenetic age by at least 2 years over 6 months?
1022. What absolute number of autologous MSCs per kilogram of body weight is required to sustainably suppress high-sensitivity CRP levels to <0.5 mg/L in patients over 60 years of age?
1023. At what cryopreservation temperature (–80°C, –150°C, –196°C) are the least losses in MSC proliferative potential recorded after 10 years of storage?
1024. What is the optimal M1/M2 macrophage ratio in a co-culture that minimizes the level of the proinflammatory cytokine IL-6 after MSC infusion?
1025. What changes in the DNA methylation pattern of the TERT gene promoter in MSCs reliably correlate with a 20% elongation of telomeres after 30 passages in vitro?
1026. What donor age (in years) provides the maximum expression of the SOD2 gene in MSCs, measured 24 hours after cell isolation?
1027. What is the only chemical inhibitor of the mTOR pathway, added to the MSC culture at a concentration of 10 nM, that increases their secretion of spermidine by at least 30%?
1028. Which type of physical pretreatment (cyclic stretch vs. 5% hypoxia) increases HIF-1α gene expression in MSCs by 2-fold without decreasing their viability?
1029. What value of maximum mitochondrial respiratory capacity (OCR, pmolO₂/min/10⁶cells) in MSCs is sufficient to observe an increase in the patient's VO₂max by at least 10% over 3 months after their infusion?
1030. Which small interfering RNA cassette can reliably reduce the synthesis of TGF-β1 in MSCs without affecting TGF-β3, to reduce the risk of fibrosis after administration?
1031. What ratio of CD146⁺/CD105⁺ subpopulations in MSC preparation correlates with an increase in bone density (T-scoreDXA) by 0.5 units 12 months after intraosseous injection?
1032. What levels of miR-21 expression in MSC are threshold for suppression of the senescence marker p16^INK4a in recipient fibroblasts?
1033. What regime of electron beam irradiation (dose and power) sterilizes MSC suspension while maintaining ≥90% of their adhesive capacity?
1034. Which specific synthetic matrix (peptide hydrogel formula) increases MSC survival after subcutaneous implantation by 2 times compared to collagen gel?
1035. What is the minimum serum vitamin D₃ concentration (ng/ml) required for stable engraftment of infused MSCs in the bone marrow niche?
1036. Which HLA-G gene polymorphism in the MSC donor is associated with the greatest decrease in TNF-α levels in the recipient 7 days after therapy?
1037. Which PGE₂ secretion index (pg/ml/10⁶cells) in the MSC supernatant predicts a decrease in osteoarthritis pain by ≥30mm according to VAS 1 month after injection?
1038. What is the maximum allowable total volume of MSC injections (ml) into the knee joint that prevents synovitis while maintaining clinical efficacy?
1039. Which single epigenetic cocktail (a combination of vitamin C, Na-buterate, and TrichostacinA) accelerates NANOG expression in MSCs within 48 h without increasing c-Myc expression?
1040. What partial pressure of O₂ (mmHg) in culture chambers ensures minimal formation of reactive oxygen species in MSCs on the 7th day of culture?
1041. Which variant of CRISPRa activation of the Klotho gene in MSCs leads to a 2-fold increase in the secretion of α-Klotho protein 72 h after transfection?
1042. What is the optimal interval (day) between repeated intravenous infusions of MSCs that minimizes the risk of transient cytokine release syndrome?
1043. What expression of the CD73 marker on the surface of MSCs (MFI, relative units) is sufficient to suppress the proliferation of autoreactive CD4⁺ T cells by 50%?
1044. What daily dose of resveratrol (mg/kg) additionally increases the survival of transplanted MSCs by 30% in a myocardial ischemia model?
1045.

Perpetual Prompt ♾️, [09.05.2025 00:09]
What mitochondrial membrane potential index (ΔΨm, JC-1ratio) in MSCs predicts their ability to restore cardiac stroke volume by ≥10% after infarction?
1046. What is the maximum number of in vitro passages allowed for MSCs so that their γ-H2AX level does not exceed 5 foci per nucleus, ensuring the absence of genotoxicity during use?
1047. Which N-glycosylation profile of surface integrinβ1 in MSCs ensures the best adhesion to the endothelium of lung capillaries?
1048. Which one-step protocol for enrichment of MSCs with CD271 magnetic nanoparticles increases the purity of the population to 95% in 30 min without reducing cell viability?
1049. What change in the sirtuin-1 level (Western blot, relative units) after treatment of MSCs with nicotinamide riboside predicts an increase in their anti-apoptotic protection?
1050. Which single plasma marker (e.g. GDF-15, β-2-M) most accurately reflects long-term (>1year) reduction of systemic inflammation after MSC therapy?