Longevity Questions

Perpetual Prompt ♾️, [09.05.2025 00:13]
1051. What is the precise rate constant (k, event CpG⁻¹ yr⁻¹) for spontaneous demethylation of the fastest drifting sites in human hematopoietic stem cells, as measured by whole-genome bisulfite sequencing?
1052. How many megajoules of ATP per day does an adult human liver cell require to maintain DNMT1 activity, which prevents >99% of remethylation errors during DNA replication?
1053. How does the median lifespan of a mouse change if Tet2 expression is suppressed to exactly 50% of normal using CRISPR in bone marrow cells from the age of three months?
1054. What is the distribution of potential energy barrier heights (in kT) for spontaneous methylation of transcription factor gene promoters in human hippocampal neurons?
1055. What is the minimum number of rare high-energy (ΔG>20 kT) methylation events in cardiomyocytes that statistically predicts the onset of myocardial fibrosis by age 60?
1056. By what percentage does the rate of copy methylation errors in human fibroblast culture decrease when intracellular SAM levels are increased by 30% using methionine β-detoxification?
1057. How many CpG sites in the naked mole-rat genome exhibit age-dependent Δβ>0.02 over 10 years, and what is their percentage of the total number of CpGs in the same tissues in mice?
1058. What is the absolute value of τ½ (half-life) of age-related methylation drift at the ELOVL2 locus in human retina, based on longitudinal sequencing data at 10-year intervals?
1059. What proportion of extreme methylation jumps (Δβ>0.25 jump per year) are due to single double-strand DNA breaks within ±50 bp of the CpG site in skin cells?
1060. How does the energy barrier (in kT) for demethylation of the p16^INK4a promoter change with a 5-fold increase in ascorbate concentration in vitro?
1061. What percentage of CpG islands in Hydra are completely devoid of methylation marks in all cell lines, according to WGBS maps?
1062. By how many days is the average lifespan of Drosophila melanogaster extended by expression of bacterial 5-mC-DNA dialkylglyco-dioxygenase, which accelerates demethylation by 10% on average?
1063. How many megabytes of memory are required to store the complete Gumbel distribution of methylation barriers for all 28 million CpG sites in the human genome?
1064. What is the p-value for the difference in the frequency of high-energy epigenetic transitions between human somatic and germ cells based on the results of a χ² test on N=1,000,000 events?
1065. How much does the Gumbel coefficient (α) of barrier distribution in intestinal epithelium change after 12 weeks of resveratrol at a dose of 150 mg/day in humans aged 50-55 years?
1066. What is the correlation (Spearmanρ) between the level of 8-oxo-dG in DNA and the frequency of extreme methylation events in monocytes in smokers with a history of >20 years?
1067. What is the minimum dose of glucagon-like peptide 1 (GLP-1) in a mouse model that reduces the rate of age-related methylation drift in pancreatic β-cells by 15%?
1068. How many minutes does it take TET1 to remove a methyl tag from a 50-partner oligonucleotide at 37°C and pH 7.4 in vitro?
1069. What is the lifespan gain (in days) for doubling PARP1 expression if it reduces the frequency of extreme methylation events in human skin stem cells by 5%?
1070. What is the Shannon entropy of the distribution of FOXO3A promoter methyl states in lymphocytes from centenarians >100 years old?
1071. How does the area under the ROC curve for the Horvath epigenetic clock change when the 5% most noisy CpG sites exhibiting a Gumbel tail are excluded?
1072. What percentage of high-energy methylation events in rat liver are suppressed by co-administration of nicotinamide and α-ketoglutarate?
1073. What is the rate of accumulation of methylation errors (events gene year⁻¹) observed in Turritopsis dohrnii during one rejuvenation cycle?

Perpetual Prompt ♾️, [09.05.2025 00:13]
1074. By how many kilobases does the average length of unmethylated intervals of LINE-1 decrease between the ages of 20 and 70 in humans?
1075. What is the critical concentration of intracellular glutathione at which the demethylating activity of TET2 falls below 70% of normal in neuronal cell culture?
1076. What is the contribution (in %) of transcriptionally active Alu repeats to the total flux of extreme methylation transitions in the human brain after 65 years of age?
1077. To what extent does the frequency of maintenance methylation errors in the skin decrease with local application of 0.1% salmelin for 30 days in patients aged 60–65 years?
1078. What threshold (in kT) of the energy barrier ensures a halving of the number of extreme methylation events compared to the median value for kidney tissue?
1079. By how many units of biological age (DNA methylation age) does the indicator decrease in mice that received weekly infusions of purified DNMT1-α positive exosomes compared to controls?
1080. What is the maximum reduction in the coefficient of variation of the 450k CpG panel methyl profile in humans achieved by a combination of high-intensity exercise and cyclic α-ketoamide supplementation for 12 months?