Longevity Questions

Perpetual Prompt ♾️, [09.05.2025 00:41]
1261. What is the minimum amino acid sequence change in the AMA1 protein of Toxoplasma needed to prevent its binding to the receptor on human cells?
1262. Can targeted inhibition of the TgCDPK1 enzyme in latent cysts completely stop their reactivation in vivo?
1263. What threshold concentration of monoclonal antibodies against SAG1 in human plasma prevents primary infection during oral challenge?
1264. To what extent does a decrease in the expression of the human CXCR4 receptor in neurons reduce the penetration of tachyzoites into the brain (% relative to control)?
1265. What is the maximum daily intake of organoselenium compounds that is safe and simultaneously suppresses intracellular reproduction of Toxoplasma (>50% inhibition)?
1266. Does oral delivery of 20nm zinc oxide nanoparticles reduce the rate of Toxoplasma cyst formation in the mouse brain by >75%?
1267. Which microbiome change index (Shannon≥x) after three weeks of a high-polyphenol diet correlates with a 2-fold reduction in Toxoplasma load?
1268. At what serum IFN-γ level (pg/mL) does latent infection progress to an uncontrolled stage in immunocompromised patients?
1269. Which combination of HLA-DRB1 alleles provides the greatest (≥4×) reduction in the risk of congenital toxoplasmosis?
1270. Can a single infusion of SAG2A-recognizing CAR-NK cells reduce the number of cysts in human brain by ≥90%?
1271. What is the detection limit of PCR screening (DNA copies/mL) needed to detect asymptomatic carriers before cysts form?
1272. Does daily inhalation of yppm NO reduce Toxoplasma replication in human lung tissue by at least 60%?
1273. What level of the tryptophan metabolite kynurenine (µM) completely abolishes tachyzoite division in vitro?
1274. What dose (mg/kg) of TgGST inhibitor achieves 95% survival in mice in a model of acute toxoplasmosis?
1275. What number of CRISPR-induced single nucleotide substitutions in the GRA16 promoter reduces the expression of this gene by at least 80%?
1276. Can chronic expression of an artificial miRNA cassette in astrocytes reduce the cyst burden in the brain by ≥50% by week 12?
1277. What parameters of tissue oximetry (mmHg) indicate successful suppression of hypoxia around Toxoplasma cysts?
1278. By how much does the average survival time of infected mice increase (%) with monthly photodynamic therapy of the brain?
1279. What is the minimum number of magnetohyperthermia cycles (10 min each) required to destroy 90% of cysts in the hippocampus?
1280. By what percentage is the shortened telomere T/S index lengthened in lymphocytes after eradication of toxoplasma with experimental drug X?
1281. What threshold of vitamin D content (nmol/L) correlates with a 3-fold decrease in the probability of primary infection?
1282. Can a protein vaccine based on recombinant ROP18 cause ≥80% neutralization of tachyzoites in a passive neutralizing immunity test?
1283. What time interval (days) between taking the probiotic Lactobacillusrhamnosus and infection reduces parasite invasion by half-maximal inhibitory concentration?
1284. At what value of the BBB permeability coefficient (×10⁻⁸cm/s) does the liposomal preparation of doxycycline reach a bacteriostatic concentration against toxoplasma?
1285. By how many points (out of 100) does the cognitive index MoCA improve in chronically infected elderly people after a 6-month course of the antioxidant N-acetylcysteine?
1286. What is the maximum level of circulating IL-6 (pg/ml) that does not shorten the median survival of infected laboratory rats?
1287. Does a 12-week ketogenic diet reduce the density of cysts in the cerebral cortex of people with latent toxoplasmosis by more than 30%?
1288. What is the minimum inhibitory effect (IC₅₀, nM) of a new TgDHFR-TS inhibitor sufficient for approval of further preclinical trials?
1289. Does regular physical activity of ≥6METs increase hippocampal microglial activity, promoting cyst resorption (measured as % of area)?
1290. How many years on average does reducing chronic Toxoplasma inflammatory burden to hs-CRP<0.5mg/L add to life expectancy?