Longevity Questions

Perpetual Prompt ♾️, [09.05.2025 00:47]
1291. Does suppression of mTORC1 complex activity by 80 nM rapamycin analogue in human fibroblasts reduce the level of phosphorylated S6K1 by at least 80% without increasing the ROS marker (> 10%) for 72 hours?
1292. Does a single CRISPRa activation of the KLOTHO promoter in C57BL/6 mice prolong their average lifespan by at least 15% (p < 0.05)?
1293. Does six-month oral administration of 500 mg/day NMN reduce biological age according to the Horvath2023 method in human blood by at least 2 years compared to placebo?
1294. Does weekly infusion of 100 ml plasma from 18-25 year old donors maintain N-galactosylated IgG levels in 60-70 year old recipients at the level of young donors for 1 year?
1295. Does single-dose siRNA silencing of PCSK9 (300 mg) increase lifespan of male ApoE-/- mice by ≥12%?
1296. Does chronic β2-microglobulin suppression (anti-B2M antibody, 10 mg/kg monthly) reduce the rate of hippocampal volume decline in aged macaques by at least 20% per year?
1297. Does a single intra-tail vein injection of the AAV vector TERT in 20-month-old mice increase leukocyte telomeric length by ≥1 kbp after 4 months?
1298. Does a BCAA-restricted diet of 35 mg kg⁻¹ day⁻¹ reduce plasma IGF-1 levels in 40-55-year-old humans by at least 25% after 3 months?
1299. Does a three-week injection of the metabolite α-keto-glutarate (1 g/kg⋅day) increase the maximum lifespan of C. elegans by at least 25%?
1300. Does a single CRISPR deletion of p16^INK4a in mouse hematopoietic stem cells reduce the frequency of senescent cells in bone marrow by ≥50% after 6 months?
1301. Does continuous administration of 10 mg fisetin/kg/day for 8 weeks reduce the proportion of SA-β-Gal⁺ cells in adipose tissue of 70-80 year old humans by at least 30%?
1302. Does weekly administration of MOTS-c peptide (15 mg/kg) increase endurance (VO₂max) in old mice (24 months) by ≥15% after 2 months?
1303. Does the combination of niacinamide 500 mg + betaine 1 g per day suppress the level of H3K9me3 in human oral epithelial cells by ≥15% in 3 months?
1304. Does a single integration of the SIRT6-V5 gene into human induced stem cells lead to an extension of their replicative potential by more than twofold the number of divisions?
1305. Does three courses of the combination D+Q (dasatinib 100 mg + quercetin 1000 mg, 3 days in a row monthly) reduce the average level of SASP-factor IL-6 in the plasma of people 65-75 years old by ≥40%?
1306. Does 6-month treatment with metformin 1500 mg/day increase insulin sensitivity (HOMA-IR) in non-diabetics 50-60 years old by ≥25%?
1307. Does a single suppressive mutation of the MYC gene in mice reduce tumor incidence by 50% by ≥40% at 30 months?
1308. Does daily 6-hour hypoxic exposure (FiO₂=14%) increase endogenous HIF-1α expression in human lymphocytes by ≥2-fold after 4 weeks?
1309. Does sterile microbiota transfer from 18-month-old long-lived donor mice to normal age-matched mice extend maximal lifespan by ≥10%?
1310. Does an 8-week 16/8 intermittent fasting program reduce DunedinPACE (Dunedin PACE) methylation to ≥0.05 units?
1311. Does daily thyrostatin (0.2 mg/kg) increase leukocyte telomere length in mice by ≥0.5 kbp over 6 months?
1312. Does CD38 inhibitor 78c (30 mg/kg) suppress NAD⁺ catabolism and reduce muscle loss in aged mice (24 months) by ≥20% over 6 months?
1313. Does genetic enhancement of NRF2 expression in mouse liver cells reduce 4-HNE marker levels by ≥60% after 2 months?
1314. Does daily supplementation with 1 g eicosapentaenoic acid (EPA) reduce telomere length shortening in human leukocytes by ≥10% per year?
1315. Does daily supplementation with sulforaphane (30 mg) increase endogenous NQO1 expression in human neutrophils by ≥50% per month?
1316. Does 2-week suppression of GDF11 antiserum increase bone mineral density (DXA) in aged mice by >15%?
1317. Does a 4-week cold immersion protocol (10°C, 3 min, 5 times a week) reduce serum FGF21 protein levels in humans by ≥25%?

Perpetual Prompt ♾️, [09.05.2025 00:47]
1318. Does monthly administration of 17-α-estradiol (14 mg/kg) reduce visceral fat levels in old male mice by ≥20%?
1319. Does editing of the promotoraPGC-1α gene in mice increase cardiac mitochondrial content by ≥30% after 8 weeks?
1320. Does monthly infusion of recombinant klotho-β (0.1 mg/kg) prolong the lifespan of male DBA/2 mice by ≥12%?